| Expression System | CHO |
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| Protein Tag | C-FLAG/C-His |
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| Purity | > 95% by SDS-PAGE under reducing conditions |
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| Endotoxin | < 1 EU per ug by the LAL method |
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| Activity | Positive |
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| Conjugate | Unconjugated |
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| Species | Human |
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| Accession Number | P07687/AAD13932.1 |
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| Protein Sequence | Ala43-Met557/Leu23-Thr529 |
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| Molecular Weight | The 522 amino acid recombinant human HexA protein has the predicted molecular weight of 60 kD. The DTT-reduced protein migrates at approximately 65 kD on SDS-PAGE. The 522 amino acid recombinant human HexB protein has the predicted molecular weight of 59.8 kD. The DTT-reduced protein migrates at approximately 65 kD on SDS-PAGE. |
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| Formulation | 25 mM Tris, 150 mM NaCl and 20%(v/v) Glycerol, pH 7.5 (0.2 μm filtered) |
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| Concentration | 25µg size is bottled at 0.2mg/mL concentration. 100 µg and 500 µg size is bottled at lot specific concentration. |
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| Purification | Affinity M2/Ni |
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| Applications | ELISA |
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| Format | Liquid |
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| Reconstitution | NA |
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| Shipping Condition | Cold Pack |
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| Storage/Shelf Life | An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. This stock solution should be aliquoted and stored at -70°C to minimize the freeze/thaw cycles. |
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| Background Information | The HEXB gene provides instructions for producing the beta subunit of two related enzymes, beta-hexosaminidase A and beta-hexosaminidase B, which are essential for breaking down certain molecules in the brain and spinal cord. These enzymes, found in lysosomes within nerve cells, are crucial for the degradation of fatty compounds like sphingolipids and complex sugars. Mutations in the HEXB gene can lead to GM2 gangliosidoses, a group of neurodegenerative disorders characterized by the accumulation of GM2 ganglioside in nerve cells. |
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