| Expression System | CHO |
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| Protein Tag | C-Fc |
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| Purity | > 95% by SDS-PAGE under reducing conditions |
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| Endotoxin | < 1 EU per ug by the LAL method |
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| Activity | Positive |
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| Conjugate | Unconjugated |
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| Species | Human |
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| Accession Number | Q15526 |
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| Protein Sequence | Val98-Val265 |
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| Molecular Weight | The 406 amino acid human SURF1 (Val98-Val265) with C-terminus human IgG1-Fc tag has the predicted molecular weight of 45.3 kD. The DTT-reduced protein migrates at approximately 50 kD on SDS-PAGE |
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| Formulation | PBS with 5mM DTT, pH 7.4 (0.2 μm filtered) |
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| Concentration | 25µg size is bottled at 0.1mg/mL concentration. 100 µg and 500 µg size is bottled at lot specific concentration. |
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| Purification | Protein A |
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| Applications | ELISA |
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| Format | Liquid |
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| Reconstitution | NA |
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| Shipping Condition | Cold Pack |
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| Storage/Shelf Life | An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. This stock solution should be aliquoted and stored at -70°C to minimize the freeze/thaw cycles. |
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| Background Information | The SURF1 protein in humans is involved in the assembly of cytochrome c oxidase (COX), also known as complex IV, which is a key enzyme in oxidative phosphorylation. SURF1 is located within the inner mitochondrial membrane and is part of a larger complex called the MITRAC complex. Mutations in the SURF1 gene are associated with Leigh syndrome and some forms of Charcot-Marie-Tooth disease. |
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