| Expression System | HEK293 |
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| Protein Tag | C-His |
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| Purity | > 95% by SDS-PAGE under reducing conditions |
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| Endotoxin | < 1 EU per ug by the LAL method |
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| Activity | Positive |
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| Conjugate | Unconjugated |
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| Species | Human |
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| Accession Number | Q15526 |
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| Protein Sequence | Val98-Val265 |
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| Molecular Weight | The 355 amino acid recombinant Human SUMF1 has the predicted molecular weight of 38.8 kD. The DTT-reduced protein migrates at approximately 45 and 40 kD on SDS-PAGE |
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| Formulation | 25 mM HEPES, 150 mM NaCl, 10% Glycerol, pH 7.4 |
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| Concentration | 25µg size is bottled at 0.1mg/mL concentration. 100 µg and 500 µg size is bottled at lot specific concentration. |
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| Purification | Affinity Ni-NTA |
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| Applications | ELISA |
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| Format | Liquid |
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| Reconstitution | NA |
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| Shipping Condition | Cold Pack |
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| Storage/Shelf Life | An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. This stock solution should be aliquoted and stored at -70°C to minimize the freeze/thaw cycles. |
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| Background Information | Human SUMF1 is a protein called Sulfatase Modifying Factor 1 or Formylglycine-generating enzyme (FGE), essential for activating sulfatases. It's a glycosylated enzyme found in the endoplasmic reticulum (ER) and is secreted from cells. SUMF1 modifies sulfatases by converting cysteine residues into C-alpha-formylglycine, which is necessary for their activity. Mutations in the SUMF1 gene lead to multiple sulfatase deficiency (MSD), a lysosomal storage disorder. |
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