| Expression System | CHO |
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| Protein Tag | C-His-Avi |
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| Purity | > 95% by SDS-PAGE under reducing conditions |
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| Endotoxin | < 1 EU per ug by the LAL method |
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| Activity | Positive |
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| Conjugate | Biotin |
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| Species | Human |
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| Accession Number | Q8WVN6-1 |
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| Protein Sequence | Gln29-Gly145 |
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| Molecular Weight | The protein has a predicted molecular weight of 16kDa and migrates at approximately 20kDa on SDS-PAGE with DTT-reduced condition. |
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| Formulation | PBS buffer, pH7.4, 0.22 µm filtered |
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| Concentration | 25µg size is bottled at 0.2mg/mL concentration. 100 µg and 500 µg size is bottled at lot specific concentration. |
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| Purification | Affinity Ni-NTA |
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| Applications | ELISA |
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| Format | Liquid |
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| Reconstitution | NA |
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| Shipping Condition | Cold Pack |
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| Storage/Shelf Life | An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. This stock solution should be aliquoted and stored at -70°C to minimize the freeze/thaw cycles. |
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| Background Information | Human SECTM1 (secreted and transmembrance 1) is a type I transmembrane glycoprotein consisted of a 28 aa signal sequence, a 117 aa extracellular domain (ECD) with one potential N?linked glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic sequence. It is localized in the Golgi apparatus and exists as a transmembrane and a soluble form. SECTM1 protein expression is detected in some myeloid cells, such as stimulated monocytes, immature monocyte?derived dendritic cells, and granulocytes. In the thymus, it is also expressed by epithelia and fibroblasts. Its expression is stimulated by IFN-gamma. Although its function is not well understood, the soluble N-terminus form of human SECTM1 demonstrates that it is a costimulatory ligand for human CD4 and CD8 T cell proliferation and cytokine production in CD7-dependent manner. |
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