| Expression System | E.coli |
|---|
| Protein Tag | C-Myc-Avi-V5-FLAG-HA-His |
|---|
| Purity | > 95% by SDS-PAGE under reducing conditions |
|---|
| Endotoxin | < 1 EU per ug by the LAL method |
|---|
| Activity | Positive |
|---|
| Conjugate | Unconjugated |
|---|
| Species | S. japonicum |
|---|
| Accession Number | AAB59203 |
|---|
| Protein Sequence | Met1-Arg228 |
|---|
| Molecular Weight | The 321 amino acid protein has a predicted molecular weight of 35.9 kDa. The protein migrates at approximately 35 kDa on SDS-PAGE with DTT-reduced condition. Some smaller bands below 30 kDa might correspond to the early translational termination products. |
|---|
| Formulation | PBS buffer, pH7.4, 0.22 µm filtered |
|---|
| Concentration | 25µg size is bottled at 0.1mg/mL concentration. 100 µg and 500 µg size is bottled at lot specific concentration. |
|---|
| Purification | Affinity Ni-NTA |
|---|
| Applications | ELISA |
|---|
| Format | Liquid |
|---|
| Reconstitution | NA |
|---|
| Shipping Condition | Cold Pack |
|---|
| Storage/Shelf Life | An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. This stock solution should be aliquoted and stored at -70°C to minimize the freeze/thaw cycles. |
|---|
| Background Information | A "Multi-Tag Control Protein" containing multiple affinity tags, like GST-Myc-Avi-V5-FLAG-HA-His, is used as a positive control in Western blotting and ELISAs to verify the efficiency of the protocol and antibody recognition. This control protein demonstrates that your experiment is working correctly and that your antibody can detect the protein of interest, which may not be present in your samples. |
|---|
