Product Details

Ni Focurose FF (TED) is a purification method that utilizes the interaction between Ni2+ and certain amino acids (primarily histidine, cysteine, and tryptophan) on the side chains of proteins for separation and purification. It is suitable for the purification of His-tagged proteins and other biomolecules that interact with Ni2+. The strong chelating property of Ni2+ allows direct application in the purification of His-tagged proteins expressed in eukaryotic secretion systems, tolerating higher levels of reducing agents and chelators without the need for sample pretreatment. The resin can be easily cleaned and regenerated without nickel stripping, enabling direct NaOH cleaning.