Product Details

Ni Focurose FF(IMAC) utilizes the interaction between Ni2+ and certain amino acids on the side chains of proteins (primarily histidine, cysteine, and tryptophan) for separation and purification. It is suitable for the separation and purification of His-tagged proteins and other biomolecules that interact with Ni2+.

Features
Fast and simple (one-step purification)
Wide applicability and easy operation, suitable for gravity columns and prepacked columns (peristaltic pump or chromatography systems)
Multiple choices, can chelate various metal ions (such as Cu2+, Zn2+, Fe2+, Co2+, Ca2+, etc.) for use
Compared to Ni Focurose FF (IDA), Ni2+ detachment is low and it has broad reagent compatibility
Note: When chelating Ca2+, avoid using phosphate buffer (as it may form precipitates)