| Expression System | HEK293 |
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| Protein Tag | C-His |
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| Purity | > 95% by SDS-PAGE under reducing conditions |
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| Endotoxin | < 1 EU per ug by the LAL method |
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| Activity | Positive |
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| Conjugate | Unconjugated |
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| Species | Virus |
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| Accession Number | QHD43416.1 |
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| Protein Sequence | Arg319-Phe541 |
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| Molecular Weight | The 236 amino acid protein has the predicted molecular weight of 26.6 kDa. The protein migrates at approximately 30-35 kDa on SDS-PAGE with DTT-reduced condition. |
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| Formulation | PBS buffer, pH7.4, 0.22 µm filtered |
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| Concentration | 25µg size is bottled at 0.1mg/mL concentration. 100 µg and 500 µg size is bottled at lot specific concentration. |
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| Purification | Affinity Ni-NTA |
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| Applications | ELISA |
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| Format | Liquid |
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| Reconstitution | NA |
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| Shipping Condition | Cold Pack |
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| Storage/Shelf Life | An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. This stock solution should be aliquoted and stored at -70°C to minimize the freeze/thaw cycles. |
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| Background Information | SARS-CoV-2 belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N). The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The SARS-CoV spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
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