| Expression System | E.coli |
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| Protein Tag | C-His |
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| Purity | > 95% by SDS-PAGE under reducing conditions |
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| Endotoxin | Not tested |
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| Activity | Positive |
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| Conjugate | ATTO488 |
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| Species | Aeromonas hydrophila |
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| Accession Number | M9T3R7 |
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| Protein Sequence | Ala24-Arg453 |
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| Formulation | PBS buffer, pH7.4, 0.22 µm filtered |
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| Concentration | 25µg size is bottled at 0.1mg/mL concentration. 100 µg and 500 µg size is bottled at lot specific concentration. |
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| Purification | Affinity Ni-NTA |
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| Applications | FC |
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| Format | Liquid |
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| Reconstitution | NA |
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| Shipping Condition | Cold Pack |
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| Storage/Shelf Life | An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. This stock solution should be aliquoted and stored at -70°C to minimize the freeze/thaw cycles. |
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| Background Information | FLAER (Fluorescently Labeled Inactive Aerolysin) is a recombinant, fluorescently labeled protein derived from aerolysin, a pore-forming toxin originally secreted by Aeromonas hydrophila. The version used in biomedical research is non-toxic and specifically binds to GPI-anchored proteins on the cell surface.FLAER is used primarily in flow cytometry to detect GPI-anchor deficiencies, particularly in: Paroxysmal Nocturnal Hemoglobinuria (PNH) diagnosis, GPI-anchor biosynthesis studies.FLAER binds specifically to GPI-anchored proteins like CD55 and CD59, which are absent in PNH cells. |
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